Guide Confocal Microscopy: Methods and Protocols

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Special Offers Classic Titles on Sale. Recent advances in microscope technology, labeling techniques and gene and protein manipulation methods have led to breakthroughs in our understanding of biological processes. In order to take advantage of these techniques, biologists need to understand the fundamental techniques of microscopy. The methods found here, drawn from the popular laboratory standard manual Cells: A Laboratory Manual, provide a solid course in the basics of using the microscope in a biology laboratory.

Light Microscopy 2. Preparation of Cells and Tissues for Fluorescence Microscopy 5. Nonimmunological Fluorescent Labeling of Cellular Structures 6. Motionless fast 3D scanning. Nature Methods 5: Fink, C.


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Intracellular fluorescent probe concentrations by confocal microscopy. Frank, J. A white light confocal microscope for spectrally resolved multidimensional imaging.

Reference Books in Confocal Microscopy

Fujita, K. High-resolution confocal microscopy by saturated excitation of fluorescence. Physical Review Letters Gauderon, R. Effect of a confocal pinhole in two-photon microscopy. Gopinath, S.


  1. Confocal Microscopy Methods and Protocols.
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  4. A statistical approach for intensity loss compensation of confocal microscopy images. Halbhuber, K. Modern laser scanning microscopy in biology, biotechnology and medicine. Annals of Anatomy Hepler, P.

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    Confocal fluorescence microscopy of plant cells. Protoplasma Itzkan, I. Cipolloni, P. Confocal light absorption and scattering spectroscopic microscopy monitors organelles in live cells with no exogenous labels. Automated acquisition and processing of multidimensional image data in confocal in vivo microscopy.

    Kreft, M. Focus-Drift correction in Time-Lapse confocal imaging. Annals of the New York Academy of Sciences Lamprecht, A. Structural analysis of microparticles by confocal laser scanning microscopy. Martinez-Corral, M.

    Confocal microscopy of cardiac myocytes

    Optical sectioning by two-pinhole confocal fluorescence microscopy. Micron McConnell, G. Confocal laser scanning fluorescence microscopy with a visible continuum source.

    Optics Express Michalet, X. Ultrahigh-resolution colocalization of spectrally separable point-like fluorescent probes.

    Methods In addition, nuclei in the specimen were counterstained with Hoechst Images were recorded in grayscale with an Olympus FluoView FV coupled to a BX inverted microscope using Argon-ion nanometer line , violet diode nanometers , and green helium-neon nanometers lasers. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.

    The vitality and physical properties of adherent cells grown on coverslips in Petri dishes can be determined using a combination of fluorescent stains. This protocol details a generalized procedure for staining a variety of cell types.

    Olympus Fluoview Resource Center: Recommended Books on Confocal Microscopy

    Fibroblast and epithelial cell lines derived from humans and laboratory animals produce brightly colored fluorescent specimens highlighting specific proteins in the intermediate filament network when stained. Cell lines and mammalian tissue sections derived from humans and laboratory animals, including intestine, kidney, testes, muscle, liver, and the lungs, produce brightly colored fluorescent specimens. Epithelial cell lines derived from humans and laboratory animals produce brightly colored fluorescent specimens detailing the cytokeratin intermediate filament network when stained with keratin antibodies.

    A majority of the common tissue sections derived from laboratory animals, including intestine, kidney, testes, muscle, liver, and the lungs, produce brightly colored fluorescent specimens detailing a wide variety of anatomical features when stained.